Reply to "Calibration Technologies for Correct Determination of Epstein-Barr Virus, Human Herpesvirus 6 (HHV-6), and HHV-8 Antiviral Drug Susceptibilities by Use of Real-Time-PCR-Based Assays".

J Clin Microbiol. 2013 Jun;51(6):2014

Authors: Bounaadja L, Piret J, Goyette N, Boivin G

PMID: 23682103 [PubMed - in process]

Calibration Technologies for Correct Determination of Epstein-Barr Virus, Human Herpesvirus 6 (HHV-6), and HHV-8 Antiviral Drug Susceptibilities by Use of Real-Time-PCR-Based Assays.

J Clin Microbiol. 2013 Jun;51(6):2013

Authors: Broccolo F, Lusso P, Malnati M

PMID: 23682102 [PubMed - in process]

A Chemical Lift-off Process: Removing Non-Specific Adsorption in an Electrochemical Epstein-Barr Virus Immunoassay.

Chemphyschem. 2013 May 16;

Authors: Stratmann L, Gebala M, Schuhmann W

Abstract
Upon contact of sensor surfaces with complex biological samples containing a variety of different proteins, non-specific adsorption hampers the high-sensitive detection of the analyte in question. To substantially decrease the impact of non-specific adsorption at thiol-based self-assembled monolayers, a chemical lift-off process is introduced. A sequence of local hydrolysis of isooctyl 3-mercaptopropionate, covalent binding of an antigen against the Epstein-Barr virus (EBV), stepwise incubation with a serum sample possibly containing the EBV antibody and an enzyme-labeled anti-human antibody is completed with a lift-off by integral hydrolysis of the remaining ester groups at the self-assembled monolayer. The cleavage of the ester removes any non-specifically bound protein during a following stringent washing step. The substantial improvement of the detection limit of an electrochemical immunoassay against EBV using native recombinant antigens, their immobilization after local deprotection using a scanning electrochemical microscope (SECM) and the local read-out using the generator-collector mode of SECM with redox cycling amplification demonstrates the successful application of the proposed lift-off procedure.

PMID: 23681905 [PubMed - as supplied by publisher]